expression of endoderm and hepatic specific genes after in vitro differentiation of human embryonic stem cells

Authors

حسین بهاروند

hossein baharvand سیدمحمود هاشمی

seyed mahmoud hashemi سعید کاظمی آشتیانی

saeid kazemi ashtiani

abstract

background: human embryonic stem cells (hesc), which are derived from the inner cell mass of the blastocysts, have been considered to be pluripotent cells. in this study we examine the differentiating potential of hesc into hepatocytes by characterization of the expression of endoderm and liver-specific genes. methods: hesc were cultivated in suspension to form aggregates, the embryoid bodies. they were allowed to outgrowth on the plated culture with the stepwise addition of growth factors such as acidic fibroblast growth factor (afgf), hepatocyte growth factor and oncostatin m into the culture medium. the expressions of endodermal and liver specific genes such as hepatocyte nuclear factor 3β , alpha-fetoprotein (afp), albumin (alb), cytokeratin 8 (ck-8), ck-18, transthyretin, glucose 6-phosphatase and tyrosine aminotransferase were analyzed by reverse transcription-polymerase chain reaction (rt-pcr). the expressions of alb and ck-18 in the cytoplasm were analyzed by immunohistochemistry. results: the immunoblotting and chemiluminescence of the conditioned media indicated the secretion of alb and afp. rt-pcr analysis revealed that hepatic gene expression related to early and late-stage liver development were enhanced through in vitro differentiation of hesc. conclusion: our results showed the expression of endoderm and hepatic specific genes after in vitro differentiation of hesc into hepatocyte-like cells through addition of various growth factors in three dimensional culture systems (collagen type i). hesc could be a new potential source of hepatocyte for transplantation in patients with liver failure.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Expression of Endoderm and Hepatic Specific Genes after in vitro Differentiation of Human Embryonic Stem Cells

Background: Human embryonic stem cells (hESC), which are derived from the inner cell mass of the blastocysts, have been considered to be pluripotent cells. In this study we examine the differentiating potential of hESC into hepatocytes by characterization of the expression of endoderm and liver-specific genes. Methods: hESC were cultivated in suspension to form aggregates, the embryoid bodies. ...

full text

Expression of Endoderm and Hepatic Specific Genes after in vitro Differentiation of Human Embryonic Stem Cells

Background: Human embryonic stem cells (hESC), which are derived from the inner cell mass of the blastocysts, have been considered to be pluripotent cells. In this study we examine the differentiating potential of hESC into hepatocytes by characterization of the expression of endoderm and liver-specific genes. Methods: hESC were cultivated in suspension to form aggregates, the embryoid bodies. ...

full text

Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells

Objective(s): In some previous studies, the extract of embryonic carcinoma cells (ECCs) and embryonic stem cells (ESCs) have been used to reprogram somatic cells to more dedifferentiated state. The aim of this study was to investigate the effect of mouse ESCs extract on the expression of some pluripotency markers in human adipose tissue-derived stem cells (ADSCs). Materials and Methods: Human A...

full text

Differentiation of human embryonic stem cells into neurons

Human embryonic stem (ES) cells are undifferentiated pluripotent cells derived from the inner cell mass of blastocyst stage embryos. These unique cell lines have the potential to form virtually any cell type in the body and can be propagated in vitro indefinitely in an undifferentiated state. These cells are capable of forming embryoid bodies (EB) that contain cells from all three embryonic lin...

full text

Differentiation of human embryonic stem cells into neurons

Human embryonic stem (ES) cells are undifferentiated pluripotent cells derived from the inner cell mass of blastocyst stage embryos. These unique cell lines have the potential to form virtually any cell type in the body and can be propagated in vitro indefinitely in an undifferentiated state. These cells are capable of forming embryoid bodies (EB) that contain cells from all three embryonic lin...

full text

Comparing the Expression Levels of Alkaline Phosphatase, Gfra1, Lin28, and Sall4 Genes in Embryonic Stem Cells, Spermatogonial Stem Cells, and Embryonic Stem-Like Cells in Mice

Background and purpose: Spermatogenesis is a well-organized process that is influenced by a variety of factors. Alkaline phosphatase, and Gfra1, Lin28, and Sall4 genes are among the key players in this interconnected process. This study aimed to investigate the expression levels of Gfra1, Lin28, and Sall4 genes in embryonic, spermatogonial, and embryonic stem-like (ES-like) cells in mice. Mate...

full text

My Resources

Save resource for easier access later


Journal title:
iranian biomedical journal

جلد ۱۰، شماره ۳، صفحات ۱۱۷-۱۲۴

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023